SDC9 Dehalococcoides sp. (DHC)

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CHEMICAL ENGINEERING FOR REMEDIATION & ENVIRONMENTAL SUSTAINABILITY

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CHEMICAL ENGINEERING FOR REMEDIATION & ENVIRONMENTAL SUSTAINABILITY

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C.E.R.E.S. Offers Dehalococcoides Mccartyi Consortium

Engineered Bacteria for Enhanced Bioremediation of Chlorinated Solvents and other halogenated aliphatic pollutants.

The SDC‐9 culture is a pathogen‐free, non‐genetically altered microbial consortium capable of biologically degrading halogenated aliphatic pollutants including , 1,1,1‐TCA, 1,1,2‐TCA, 1,1‐DCA, 1,2‐DCA, 1,2‐DBE, TeCA, CT, CF, PCE, TCE, DCE, VC, and Freon 113 (1,2‐dichloro‐1,2‐difluoroethane), and also mixtures thereof. Molecular biological analyses of the SDC‐9 culture has demonstrated that the culture has at least four closely‐related strains of Dehalococcoides sp. bacteria which is the only bacterial genus known to degrade chlorinated ethenes completely to ethene. In addition, the culture contains other known pollutant degrading bacteria including Desulfitobacterium and Desulfovibrio strains.

It has now been successfully applied more than 3,000 times, and hundreds of thousands of liters have been delivered to sites throughout the United States.

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Spec Sheet

Benefits Include

Rapidly improves biotic degradation of halogenated aliphatic compounds.
Supplements native bacteria and accelerates bacteria colony proliferation.
Economical solution compared to other available products.
Submicron size allows for effective distribution during injection applications.
Use in conjunction with ZVI, BGCR, BGCR-S, Lactate, or other donors.
Utilize ZerO Oxygen Scavenger to prep injectate water and achieve rapid negative ORP and zero dissolved oxygen.
Bacteria shipped overnight to sites for rapid deployment.

Dehalococcoides MccArtyi (DHC)

Electron micrograph of D. mccartyi BTF08 in pure culture (A) and in co-culture with S. multivorans (B, C), where D. mccartyi BTF08 exhibits the hitherto unknown barrel-like morphology. White arrows indicate flagella and yellow arrows indicate ring-shaped septum of D. mccartyi cells.

The ISME Journal (ISME J) ISSN 1751-7370 (online) ISSN 1751-7362 (print)

Specifications and Limits

Specification	Limit
Cell Count:	> 1 x 10^11 cells/liter
Recommended dosage:	1 x 10^7 cells/liter/injection
pH Value	6-8 approx.
Packaging	19 liter chilled canisters
Appearance	greenish and cloudy

Optimal Dosage Discussion

The optimal dosage of Dehalococcoides (DHC) culture in an aquifer for effective anaerobic bioremediation of chlorinated solvents (e.g., PCE, TCE, VC) depends on several factors, including:

Contaminant Concentration: Higher concentrations of chlorinated compounds may require more DHC cells.
Aquifer Conditions: pH, temperature, redox potential, and the presence of competing electron acceptors (e.g., sulfate, nitrate) can influence DHC activity.
Electron Donor Availability: DHC requires an electron donor (e.g., hydrogen, lactate, or acetate) for reductive dehalogenation.

Typical Dosage Range: The optimal dosage of DHC in an aquifer typically ranges from 10⁶ to 10⁸ cells per liter (cells/L) of groundwater or aquifer material. This range is based on field and laboratory studies for effective dechlorination of chlorinated ethenes (e.g., PCE, TCE, VC).

For low to moderate contamination levels (e.g., TCE concentrations < 1 mg/L), a dosage of 10⁶ to 10⁷ cells/L is often sufficient.
For high contamination levels (e.g., TCE concentrations > 1 mg/L), a dosage of 10⁷ to 10⁸ cells/L may be required to ensure complete dechlorination to ethene.

Applicable Target Compounds:

Petroleum Hydrocarbons:

BTEX-Benzene, toluene, ethylbenzene, xylenes

Tert Butyl Alcohol (TBA)

MTBE-Methyl tert-butyl-ether

GRO-Gasoline Range Organics

DRO-Disel Range Organics

ORO-Oil Range Organics

Styrene

Polyaromatic Hydrocarbons (PAHs):

Anthracene

Naphthalene

Phenanthrene

Pyrene Creosote and others

Chlorinated Compounds:

Chlorobenzenes

Chemical Supplier for Remediation Projects

Sodium, Calcium, and Ethyl Lactate
Lactose, Glycerol, and other donors
Need carbon sources for trench projects? No problem!
Ferrous Sulfate Mono and Hepta-hydrate
Magnesium Oxide
Magnesium Sulfate
Phosphate Chemistries
Carbon sources
Iron based chemistries
Carbonate and other Buffers for pH Control

SDC9 Dehalococcoides sp. (DHC)

C.E.R.E.S. Offers Dehalococcoides Mccartyi Consortium

Engineered Bacteria for Enhanced Bioremediation of Chlorinated Solvents and other halogenated aliphatic pollutants.

Spec Sheet

Spec Sheet

Spec Sheet

Spec Sheet

Benefits Include

Dehalococcoides MccArtyi (DHC)

Specifications and Limits

Optimal Dosage Discussion

Petroleum Hydrocarbons:

Polyaromatic Hydrocarbons (PAHs):

Chlorinated Compounds:

Chemical Supplier for Remediation Projects

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